3hqr

X-ray diffraction
2Å resolution

PHD2:Mn:NOG:HIF1-alpha substrate complex

Released:

Function and Biology Details

Reaction catalysed:
Hypoxia-inducible factor-L-proline + 2-oxoglutarate + O(2) = hypoxia-inducible factor-trans-4-hydroxy-L-proline + succinate + CO(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Egl nine homolog 1 Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 27.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9GZT9 (Residues: 181-426; Coverage: 58%)
Gene names: C1orf12, EGLN1, PNAS-118, PNAS-137
Sequence domains: 2OG-Fe(II) oxygenase superfamily
Structure domains: q2cbj1_9rhob like domain
Hypoxia-inducible factor 1-alpha Chain: S
Molecule details ›
Chain: S
Length: 17 amino acids
Theoretical weight: 2.03 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q16665 (Residues: 558-574; Coverage: 2%)
Gene names: BHLHE78, HIF1A, MOP1, PASD8

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 39.379Å b: 68.798Å c: 72.221Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.234 0.248
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided