3hqm

X-ray diffraction
1.74Å resolution

Structures of SPOP-Substrate Complexes: Insights into Molecular Architectures of BTB-Cul3 Ubiquitin Ligases: SPOPMATHx-CiSBC2

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Speckle-type POZ protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 145 amino acids
Theoretical weight: 16.49 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43791 (Residues: 28-166; Coverage: 37%)
Gene name: SPOP
Sequence domains: MATH domain
Structure domains: Apoptosis, Tumor Necrosis Factor Receptor Associated Protein 2; Chain A
Transcriptional repressor cubitus interruptus Chains: C, D
Molecule details ›
Chains: C, D
Length: 12 amino acids
Theoretical weight: 1.31 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
UniProt:
  • Canonical: P19538 (Residues: 1356-1367; Coverage: 1%)
Gene names: CG2125, ci, ci-D

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P1
Unit cell:
a: 44.281Å b: 48.1Å c: 49.86Å
α: 63° β: 64.04° γ: 62.89°
R-values:
R R work R free
0.179 0.177 0.218
Expression systems:
  • Escherichia coli
  • Not provided