› 3h91

X-ray diffraction
1.5Å resolution

Crystal structure of the complex of human chromobox homolog 2 (CBX2) and H3K27 peptide

Released:
DOI: 10.2210/pdb3h91/pdb
PDB 3h91 coloured by chain and viewed from the front.
PDB 3h91 coloured by chain and viewed from the side.
PDB 3h91 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Recognition and specificity determinants of the human cbx chromodomains.
Kaustov L, Ouyang H, Amaya M, Lemak A, Nady N, Duan S, Wasney GA, Li Z, Vedadi M, Schapira M, Min J, Arrowsmith CH
J Biol Chem 286 521-9 (2011)
PMID: 21047797

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Chromobox protein homolog 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 54 amino acids
Theoretical weight: 6.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14781 (Residues: 9-62; Coverage: 10%)
Gene name: CBX2
Sequence domains: Chromo (CHRromatin Organisation MOdifier) domain
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)
Histone H3 Chains: C, D
Molecule details ›
Chains: C, D
Length: 15 amino acids
Theoretical weight: 1.52 KDa
Source organism: Xenopus laevis
Expression system: Not provided
UniProt:
  • Canonical: Q92133 (Residues: 20-34; Coverage: 11%)
Gene names: H3l, Hist1h3f, h3, h3.2a, h3c8, h3c8.S, h3r, hist1h3g, hist1h3g.L, hist2h3, hist2h3c

Ligands and Environments

No bound ligands
1 modified residue:
Ligand M3L 2 x M3L

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 58.145Å b: 84.013Å c: 65.499Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.21 0.209 0.223
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

25 review citations

Biology and Physics of Heterochromatin-Like Domains/Complexes.
Singh et al. (2020)
24 more

5 mentions without citation

Recognition and specificity determinants of the human cbx chromodomains.
Kaustov et al. (2011)
4 more