X-ray diffraction
1.3Å resolution

Chimeric Glycosyltransferase for the generation of novel natural products


Function and Biology Details

Reaction catalysed:
dTDP-beta-L-4-epi-vancosamine + devancoaminyl-vancomycin = dTDP + chloroorienticin B
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
GtfA protein; dTDP-epi-vancosaminyltransferase Chain: A
Molecule details ›
Chain: A
Length: 404 amino acids
Theoretical weight: 42.11 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
  • Canonical: P96558 (Residues: 1-205; Coverage: 52%)
  • Canonical: Q6ZZJ7 (Residues: 209-393; Coverage: 47%)
Gene names: FHX34_1062, gtfA, tcp8
Sequence domains:
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: C2221
Unit cell:
a: 111.48Å b: 129.83Å c: 67.44Å
α: 90° β: 90° γ: 90°
R R work R free
0.17 0.164 0.184
Expression system: Escherichia coli BL21(DE3)