PDBe 3gyj

X-ray diffraction
0.92Å resolution

Cholesterol oxidase from Streptomyces sp. N485L mutant (0.92A)

Released:

Function and Biology Details

Reactions catalysed:
A 3-oxo-Delta(5)-steroid = a 3-oxo-Delta(4)-steroid
Cholesterol + O(2) = cholest-5-en-3-one + H(2)O(2)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cholesterol oxidase Chain: A
Molecule details ›
Chain: A
Length: 504 amino acids
Theoretical weight: 54.97 KDa
Source organism: Streptomyces sp. SA-COO
Expression system: Escherichia coli
UniProt:
  • Canonical: P12676 (Residues: 43-546; Coverage: 100%)
Gene name: choA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 51.289Å b: 73.396Å c: 63.14Å
α: 90° β: 105.15° γ: 90°
R-values:
R R work R free
0.125 0.124 0.151
Expression system: Escherichia coli