X-ray diffraction
2.96Å resolution

Crystal structure of the inactive EGFR kinase domain in complex with AMP-PNP


Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
Non-polymer only trimer (preferred)
homo dimer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Epidermal growth factor receptor Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 330 amino acids
Theoretical weight: 37.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P00533 (Residues: 696-1022; Coverage: 28%)
Gene names: EGFR, ERBB, ERBB1, HER1
Sequence domains: Protein tyrosine and serine/threonine kinase
Structure domains:
Unknown peptide Chain: X
Molecule details ›
Chain: X
Length: 11 amino acids
Theoretical weight: 954 Da
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.3
Spacegroup: P21
Unit cell:
a: 61.764Å b: 72.411Å c: 143.376Å
α: 90° β: 101.74° γ: 90°
R R work R free
0.219 0.216 0.275
Expression system: Spodoptera frugiperda