› 3gjx

X-ray diffraction
2.5Å resolution

Crystal Structure of the Nuclear Export Complex CRM1-Snurportin1-RanGTP

Released:
DOI: 10.2210/pdb3gjx/pdb
PDB 3gjx coloured by chain and viewed from the front.
PDB 3gjx coloured by chain and viewed from the side.
PDB 3gjx coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Crystal structure of the nuclear export receptor CRM1 in complex with Snurportin1 and RanGTP.
Monecke T, Güttler T, Neumann P, Dickmanns A, Görlich D, Ficner R
Science 324 1087-91 (2009)
PMID: 19389996

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Snurportin-1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 365 amino acids
Theoretical weight: 41.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95149 (Residues: 1-360; Coverage: 100%)
Gene names: RNUT1, SNUPN, SPN1
Sequence domains: Snurportin1
Structure domains: DNA ligase/mRNA capping enzyme
GTP-binding nuclear protein Ran Chains: C, F
Molecule details ›
Chains: C, F
Length: 216 amino acids
Theoretical weight: 24.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Exportin-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 1073 amino acids
Theoretical weight: 123.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P5F9 (Residues: 1-1071; Coverage: 100%)
Gene names: Crm1, Xpo1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments

4 bound ligands:
Ligand NA 2 x NA
Ligand GTP 2 x GTP
Ligand MG 2 x MG
Ligand CL 1 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 72.173Å b: 225.738Å c: 163.451Å
α: 90° β: 100.56° γ: 90°
R-values:
R R work R free
0.31 0.244 0.281
Expression system: Escherichia coli

Quick links

Citations

22 review citations

Ran GTPase: A Key Player in Tumor Progression and Metastasis.
Boudhraa et al. (2020)
21 more

22 mentions without citation

Structural Basis of Targeting the Exportin CRM1 in Cancer.
Dickmanns et al. (2015)
21 more