3gjx

X-ray diffraction
2.5Å resolution

Crystal Structure of the Nuclear Export Complex CRM1-Snurportin1-RanGTP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Snurportin-1 Chains: B, E
Molecule details ›
Chains: B, E
Length: 365 amino acids
Theoretical weight: 41.61 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O95149 (Residues: 1-360; Coverage: 100%)
Gene names: RNUT1, SNUPN, SPN1
Sequence domains: Snurportin1
Structure domains: DNA ligase/mRNA capping enzyme
GTP-binding nuclear protein Ran Chains: C, F
Molecule details ›
Chains: C, F
Length: 216 amino acids
Theoretical weight: 24.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62826 (Residues: 1-216; Coverage: 100%)
Gene names: ARA24, OK/SW-cl.81, RAN
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Exportin-1 Chains: A, D
Molecule details ›
Chains: A, D
Length: 1073 amino acids
Theoretical weight: 123.37 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P5F9 (Residues: 1-1071; Coverage: 100%)
Gene names: Crm1, Xpo1
Sequence domains:
Structure domains: Leucine-rich Repeat Variant

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 72.173Å b: 225.738Å c: 163.451Å
α: 90° β: 100.56° γ: 90°
R-values:
R R work R free
0.31 0.244 0.281
Expression system: Escherichia coli