3gjo

X-ray diffraction
2.5Å resolution

Crystal structure of human EB1 in complex with microtubule Tip localization signal peptide of MACF

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Microtubule-associated protein RP/EB family member 1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 72 amino acids
Theoretical weight: 8.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q15691 (Residues: 191-260; Coverage: 26%)
Gene name: MAPRE1
Sequence domains: EB1-like C-terminal motif
Dystonin Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 30 amino acids
Theoretical weight: 3.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q03001 (Residues: 7541-7570; Coverage: 0%)
Gene names: BP230, BP240, BPAG1, DMH, DST, DT, KIAA0728

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 45.614Å b: 44.896Å c: 74.84Å
α: 90° β: 98.57° γ: 90°
R-values:
R R work R free
0.215 0.213 0.255
Expression system: Escherichia coli