› 3gia

X-ray diffraction
2.32Å resolution

Crystal Structure of ApcT Transporter

Released:
DOI: 10.2210/pdb3gia/pdb
PDB entry 3gia coloured by chain, front view.
PDB entry 3gia coloured by chain, side view.
PDB entry 3gia coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Structure and mechanism of a Na+-independent amino acid transporter.
Shaffer PL, Goehring A, Shankaranarayanan A, Gouaux E
Science 325 1010-4 (2009)
PMID: 19608859

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176549 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized protein MJ0609 Chain: A
Molecule details ›
Chain: A
Length: 444 amino acids
Theoretical weight: 48.33 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q58026 (Residues: 1-435; Coverage: 100%)
Gene name: MJ0609
Sequence domains: Amino acid permease
Structure domains: Amino acid/polyamine transporter I

Ligands and Environments

2 bound ligands:
Ligand D10 6 x D10
Ligand BCN 1 x BCN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: C2
Unit cell:
a: 113.482Å b: 99.499Å c: 72.693Å
α: 90° β: 117.71° γ: 90°
R-values:
R R work R free
0.204 0.203 0.233
Expression system: Escherichia coli

Quick links

Citations

47 review citations

SLC6 neurotransmitter transporters: structure, function, and regulation.
Kristensen et al. (2011)
46 more

33 mentions without citation

Physiology and pathophysiology of SLC12A1/2 transporters.
Markadieu et al. (2014)
32 more