› 3g7w

X-ray diffraction
1.75Å resolution

Islet Amyloid Polypeptide (IAPP or Amylin) Residues 1 to 22 fused to Maltose Binding Protein

Released:
DOI: 10.2210/pdb3g7w/pdb
PDB 3g7w coloured by chain and viewed from the front.
PDB 3g7w coloured by chain and viewed from the side.
PDB 3g7w coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Atomic structures of IAPP (amylin) fusions suggest a mechanism for fibrillation and the role of insulin in the process.
Wiltzius JJ, Sievers SA, Sawaya MR, Eisenberg D
Protein Sci. 18 1521-30 (2009)
PMID: 19475663

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Islet amyloid polypeptide; Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 42.93 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEX9 (Residues: 27-392; Coverage: 99%)
  • Canonical: P10997 (Residues: 34-55; Coverage: 33%)
Gene names: IAPP, JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC
Ligand GLC 3 x GLC
2 bound ligands:
Ligand SO4 20 x SO4
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41212
Unit cell:
a: 84.495Å b: 84.495Å c: 144.292Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.194
Expression system: Escherichia coli

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Citations

20 review citations

Peptide-Based Molecular Strategies To Interfere with Protein Misfolding, Aggregation, and Cell Degeneration.
Armiento et al. (2020)
19 more

1 mention without citation

Atomic structures of IAPP (amylin) fusions suggest a mechanism for fibrillation and the role of insulin in the process.
Wiltzius et al. (2009)