3fwe

X-ray diffraction
2.3Å resolution

Crystal Structure of the Apo D138L CAP mutant

Released:
Source organism: Escherichia coli K-12
Primary publication:
Structure of apo-CAP reveals that large conformational changes are necessary for DNA binding.
Proc. Natl. Acad. Sci. U.S.A. 106 16604-9 (2009)
PMID: 19805344

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-activated global transcriptional regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 210 amino acids
Theoretical weight: 23.67 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P43212
Unit cell:
a: 67.996Å b: 67.996Å c: 230.113Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.227 0.277
Expression system: Escherichia coli