X-ray diffraction
2.35Å resolution

Crystal structure of GDNF-GFRalpha1 complex

Source organisms:
Primary publication:
Comparison of GFL-GFRalpha complexes: further evidence relating GFL bend angle to RET signalling.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 65 551-8 (2009)
PMID: 19478429

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
GDNF family receptor alpha-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 281 amino acids
Theoretical weight: 31.11 KDa
Source organism: Rattus norvegicus
  • Canonical: Q62997 (Residues: 145-425; Coverage: 63%)
Gene names: Gdnfra, Gfra1, Retl1, Trnr1
Sequence domains: GDNF/GAS1 domain
Glial cell line-derived neurotrophic factor Chains: B, D
Molecule details ›
Chains: B, D
Length: 134 amino acids
Theoretical weight: 15.1 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P39905 (Residues: 78-211; Coverage: 70%)
Gene name: GDNF
Sequence domains: Transforming growth factor beta like domain
Structure domains: Cystine-knot cytokines

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 45.2Å b: 84.1Å c: 179.2Å
α: 90° β: 96.1° γ: 90°
R R work R free
0.228 0.226 0.28
Expression system: Spodoptera frugiperda