3fk2

X-ray diffraction
2.8Å resolution

Crystal structure of the RhoGAP domain of human glucocorticoid receptor DNA-binding factor 1

Released:
Source organism: Homo sapiens
Entry authors: Nedyalkova L, Tong Y, Tempel W, Loppnau P, Arrowsmith CH, Edwards AM, Bountra C, Weigelt J, Bochkarev A, Park H, Structural Genomics Consortium (SGC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Rho GTPase-activating protein 35 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 246 amino acids
Theoretical weight: 28.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NRY4 (Residues: 1212-1439; Coverage: 15%)
Gene names: ARHGAP35, GRF1, GRLF1, KIAA1722, P190A, p190ARHOGAP
Sequence domains: RhoGAP domain
Structure domains: Rho GTPase activation protein

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21212
Unit cell:
a: 165.025Å b: 72.408Å c: 72.863Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.226 0.223 0.273
Expression system: Escherichia coli