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X-ray diffraction
2.4Å resolution

FHIT (FRAGILE HISTIDINE TRIAD PROTEIN) IN COMPLEX WITH ADENOSINE/SULFATE AMP ANALOG

Released:
DOI: 10.2210/pdb3fit/pdb
PDB entry 3fit coloured by chain, front view.
PDB entry 3fit coloured by chain, side view.
PDB entry 3fit coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
MAD analysis of FHIT, a putative human tumor suppressor from the HIT protein family.
Lima CD, D'Amico KL, Naday I, Rosenbaum G, Westbrook EM, Hendrickson WA
Structure 5 763-74 (1997)
PMID: 9261067

Function and Biology Details

Reactions catalysed: 
Adenylyl sulfate + NH(3) = adenosine 5'-phosphoramidate + sulfate
P(1)-P(3)-bis(5'-adenosyl) triphosphate + H(2)O = ADP + AMP
Adenylyl sulfate + H(2)O = AMP + sulfate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156029 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bis(5'-adenosyl)-triphosphatase Chain: A
Molecule details ›
Chain: A
Length: 147 amino acids
Theoretical weight: 16.98 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49789 (Residues: 1-147; Coverage: 100%)
Gene name: FHIT
Sequence domains: HIT domain
Structure domains: HIT-like

Ligands and Environments

3 bound ligands:
Ligand FRU 1 x FRU
Ligand SO4 2 x SO4
Ligand A 1 x A
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6122
Unit cell:
a: 50.6Å b: 50.6Å c: 268Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.201 0.201 0.249
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Diadenosine oligophosphates (Ap(n)A), a novel class of signalling molecules?
Kisselev et al. (1998)
11 more

1 mention without citation

Tanshinones induce tumor cell apoptosis via directly targeting FHIT.
Zhou et al. (2021)