3fhp

Neutron Diffraction
2Å resolution

A neutron crystallographic analysis of a porcine 2Zn insulin at 2.0 A resolution

Released:
Source organism: Sus scrofa
Primary publication:
A neutron crystallographic analysis of T6 porcine insulin at 2.1 A resolution.
Acta Crystallogr. D Biol. Crystallogr. 65 1042-50 (2009)
PMID: 19770501

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Spacegroup: R3
Unit cell:
a: 82.849Å b: 82.849Å c: 34.173Å α: 90° β: 90° γ: 120°
R-values:
R R work R free 0.168 0.168 0.247
Expression system: Escherichia coli