3exl

X-ray diffraction
2.2Å resolution

Crystal Structure of a p53 Core Tetramer Bound to DNA

Released:
Source organism: Mus musculus
Primary publication:
Crystal structure of a p53 core tetramer bound to DNA.
OpenAccess logo Oncogene 28 325-33 (2009)
PMID: 18978813

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Cellular tumor antigen p53 Chain: A
Molecule details ›
Chain: A
Length: 197 amino acids
Theoretical weight: 22.38 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P02340 (Residues: 96-292; Coverage: 51%)
Gene names: P53, Tp53, Trp53
Structure domains: Immunoglobulin-like
5'-D(*DGP*DAP*DGP*DCP*DAP*DTP*DGP*DCP*DTP*DCP*DA)-3' Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.36 KDa
Source organism: Mus musculus
Expression system: Not provided
5'-D(*DTP*DTP*DGP*DAP*DGP*DCP*DAP*DTP*DGP*DCP*DTP*DC)-3' Chain: D
Molecule details ›
Chain: D
Length: 12 nucleotides
Theoretical weight: 3.65 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: C2
Unit cell:
a: 109.418Å b: 68.103Å c: 34.428Å
α: 90° β: 104.18° γ: 90°
R-values:
R R work R free
0.202 0.202 0.253
Expression systems:
  • Escherichia coli
  • Not provided