PDBe 3exc

X-ray diffraction
2.25Å resolution

Structure of the RNA'se SSO8090 from Sulfolobus solfataricus

Released:
Source organism: Sulfolobus solfataricus
Entry authors: Singer AU, Skarina T, Tan K, Kagan O, Onopriyenko O, Edwards AM, Joachimiak A, Yakunin AF, Savchenko A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
CRISPR-associated endoribonuclease Cas2 2 Chain: X
Molecule details ›
Chain: X
Length: 91 amino acids
Theoretical weight: 10.76 KDa
Source organism: Sulfolobus solfataricus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q97Y85 (Residues: 1-88; Coverage: 100%)
Gene names: SSO8090, cas22
Sequence domains: CRISPR associated protein Cas2
Structure domains: Alpha-Beta Plaits

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P6322
Unit cell:
a: 93.479Å b: 93.479Å c: 48.596Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.193 0.191 0.221
Expression system: Escherichia coli