PDBe 3ewe

X-ray diffraction
3.5Å resolution

Crystal Structure of the Nup85/Seh1 Complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Nucleoporin SEH1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 349 amino acids
Theoretical weight: 39.17 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P53011 (Residues: 1-349; Coverage: 100%)
Gene names: SEH1, YGL100W
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Nucleoporin NUP85 Chains: B, D
Molecule details ›
Chains: B, D
Length: 564 amino acids
Theoretical weight: 64.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P46673 (Residues: 1-564; Coverage: 76%)
Gene names: J1624, NUP85, RAT9, YJR042W

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41212
Unit cell:
a: 112.559Å b: 112.559Å c: 350.549Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.328 0.326 0.369
Expression system: Escherichia coli