3eq3

Electron Microscopy
9Å resolution

Model of tRNA(Trp)-EF-Tu in the ribosomal pre-accommodated state revealed by cryo-EM

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (9 distinct):
Elongation factor Tu 1 Chain: X
Molecule details ›
Chain: X
Length: 393 amino acids
Theoretical weight: 43.24 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0CE47 (Residues: 2-393; Coverage: 100%)
Gene names: JW3301, b3339, tufA
Sequence domains:
30S ribosomal protein S12 Chain: L
Molecule details ›
Chain: L
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
50S ribosomal protein L11 Chain: I
Molecule details ›
Chain: I
Length: 141 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7J7 (Residues: 2-142; Coverage: 99%)
Gene names: JW3946, b3983, relC, rplK
Sequence domains:
tRNA Chain: Y
Molecule details ›
Chain: Y
Length: 74 nucleotides
Theoretical weight: 23.84 KDa
Sequence domains: tRNA
Fragment h18 of the 16S rRNA Chain: A
Molecule details ›
Chain: A
Length: 9 nucleotides
Theoretical weight: 2.87 KDa
Fragment h44 of the 16S rRNA Chain: C
Molecule details ›
Chain: C
Length: 11 nucleotides
Theoretical weight: 3.6 KDa
Fragment H43-44 of the 23S rRNA Chain: B
Molecule details ›
Chain: B
Length: 48 nucleotides
Theoretical weight: 15.5 KDa
Fragment H95 of the 23S rRNA Chain: D
Molecule details ›
Chain: D
Length: 28 nucleotides
Theoretical weight: 9.09 KDa
Fragment H69 of the 23S rRNA Chain: E
Molecule details ›
Chain: E
Length: 17 nucleotides
Theoretical weight: 5.43 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-1565