3egh

X-ray diffraction
2Å resolution

Crystal structure of a complex between Protein Phosphatase 1 alpha (PP1), the PP1 binding and PDZ domains of Spinophilin and the small natural molecular toxin Nodularin-R

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
Non-polymer only hexamer
Non-polymer only trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Serine/threonine-protein phosphatase PP1-alpha catalytic subunit Chains: A, B
Molecule details ›
Chains: A, B
Length: 329 amino acids
Theoretical weight: 37.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P62136 (Residues: 7-330; Coverage: 98%)
Gene names: PPP1A, PPP1CA
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2
Neurabin-2 Chains: C, D
Molecule details ›
Chains: C, D
Length: 170 amino acids
Theoretical weight: 18.57 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O35274 (Residues: 417-583; Coverage: 20%)
Gene names: Ppp1r9b, Spino
Sequence domains:
Structure domains: Pdz3 Domain
nodularin R Chains: E, F
Molecule details ›
Chains: E, F
Length: 5 amino acids
Theoretical weight: 844 Da
Source organism: Nodularia spumigena
Expression system: Not provided

Ligands and Environments

2 bound ligands:

3 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 119.409Å b: 84.426Å c: 109.314Å
α: 90° β: 93.58° γ: 90°
R-values:
R R work R free
0.195 0.192 0.235
Expression systems:
  • Escherichia coli
  • Not provided