PDBe 3ef6

X-ray diffraction
1.8Å resolution

Crystal structure of Toluene 2,3-Dioxygenase Reductase

Released:
Source organism: Pseudomonas putida F1
Primary publication:
Structures of the multicomponent Rieske non-heme iron toluene 2,3-dioxygenase enzyme system.
OpenAccess logo Acta Crystallogr. D Biol. Crystallogr. 65 24-33 (2009)
PMID: 19153463

Function and Biology Details

Reaction catalysed:
2 reduced [2Fe-2S] ferredoxin + NAD(+) + H(+) = 2 oxidized [2Fe-2S] ferredoxin + NADH
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Toluene 1,2-dioxygenase system ferredoxin--NAD(+) reductase component Chain: A
Molecule details ›
Chain: A
Length: 410 amino acids
Theoretical weight: 42.98 KDa
Source organism: Pseudomonas putida F1
Expression system: Escherichia coli
UniProt:
  • Canonical: A5W4E9 (Residues: 1-410; Coverage: 100%)
Gene names: Pput_2878, todA
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 1 x FAD
2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: P41212
Unit cell:
a: 77.128Å b: 77.128Å c: 156.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.205 0.203 0.243
Expression system: Escherichia coli