3e9j

X-ray diffraction
3.7Å resolution

Structure of the charge-transfer intermediate of the transmembrane redox catalyst DsbB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Thiol:disulfide interchange protein DsbA Chains: B, E
Molecule details ›
Chains: B, E
Length: 189 amino acids
Theoretical weight: 21.12 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AEG4 (Residues: 20-208; Coverage: 100%)
Gene names: JW3832, b3860, dsbA, dsf, ppfA
Sequence domains: DSBA-like thioredoxin domain
Structure domains: Glutaredoxin
Disulfide bond formation protein B Chains: C, F
Molecule details ›
Chains: C, F
Length: 182 amino acids
Theoretical weight: 20.95 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A6M2 (Residues: 1-176; Coverage: 100%)
Gene names: JW5182, b1185, dsbB, roxB, ycgA
Sequence domains: Disulfide bond formation protein DsbB
Structure domains: DsbB-like

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 66.305Å b: 103.105Å c: 125.826Å
α: 90° β: 91.16° γ: 90°
R-values:
R R work R free
0.339 0.337 0.379
Expression system: Escherichia coli