3e1r

X-ray diffraction
2Å resolution

Midbody targeting of the ESCRT machinery by a non-canonical coiled-coil in CEP55

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Centrosomal protein of 55 kDa Chains: A, B
Molecule details ›
Chains: A, B
Length: 58 amino acids
Theoretical weight: 6.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q53EZ4 (Residues: 160-217; Coverage: 13%)
Gene names: C10orf3, CEP55, URCC6
Sequence domains: TSG101 and ALIX binding domain of CEP55
Structure domains: Geminin coiled-coil domain
Programmed cell death 6-interacting protein Chain: C
Molecule details ›
Chain: C
Length: 13 amino acids
Theoretical weight: 1.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q8WUM4 (Residues: 797-809; Coverage: 2%)
Gene names: AIP1, ALIX, KIAA1375, PDCD6IP

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 54.585Å b: 61.15Å c: 88.542Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.248
Expression systems:
  • Escherichia coli
  • Not provided