3e0d

X-ray diffraction
4.6Å resolution

Insights into the Replisome from the Crystral Structure of the Ternary Complex of the Eubacterial DNA Polymerase III alpha-subunit

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase III subunit alpha Chains: A, B
Molecule details ›
Chains: A, B
Length: 1220 amino acids
Theoretical weight: 137.61 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9XDH5 (Residues: 1-1220; Coverage: 100%)
Gene name: dnaE
Sequence domains:
DNA substrate template strand Chains: C, E
Molecule details ›
Chains: C, E
Length: 27 nucleotides
Theoretical weight: 8.29 KDa
Source organism: Thermus aquaticus
Expression system: Not provided
DNA substrate primer strand Chains: D, F
Molecule details ›
Chains: D, F
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Thermus aquaticus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A, APS BEAMLINE 24-ID-C
Spacegroup: P41
Unit cell:
a: 149.546Å b: 149.546Å c: 163.338Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.287 0.287 0.271
Expression systems:
  • Escherichia coli
  • Not provided