› 3dxb

X-ray diffraction
2.2Å resolution

Structure of the UHM domain of Puf60 fused to thioredoxin

Released:
DOI: 10.2210/pdb3dxb/pdb
PDB entry 3dxb coloured by chain, front view.
PDB entry 3dxb coloured by chain, side view.
PDB entry 3dxb coloured by chain, top view.
Source organisms:
Primary publication:
Dimerization and protein binding specificity of the U2AF homology motif of the splicing factor Puf60.
Corsini L, Hothorn M, Stier G, Rybin V, Scheffzek K, Gibson TJ, Sattler M
J Biol Chem 284 630-639 (2009)
PMID: 18974054

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-141966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60; Thioredoxin 1 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 222 amino acids
Theoretical weight: 24.67 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: P0AA27 (Residues: 1-109; Coverage: 100%)
  • Canonical: Q9UHX1 (Residues: 460-559; Coverage: 18%)
Gene names: ECs4714, FIR, PUF60, ROBPI, SIAHBP1, Z5291, trxA
Sequence domains: Thioredoxin
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 4 x CL
Ligand EDO 1 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 75.12Å b: 89.43Å c: 299.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.211 0.271
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Far upstream element binding protein 1: a commander of transcription, translation and beyond.
Zhang et al. (2013)
3 more

7 mentions without citation

Cancer-relevant splicing factor CAPERĪ± engages the essential splicing factor SF3b155 in a specific ternary complex.
Loerch et al. (2014)
6 more