3dwl

X-ray diffraction
3.78Å resolution

Crystal Structure of Fission Yeast Arp2/3 Complex Lacking the Arp2 Subunit

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Actin-related protein 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 427 amino acids
Theoretical weight: 47.43 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: P32390 (Residues: 1-427; Coverage: 100%)
Gene names: SPAC630.03, act2
Sequence domains: Actin
Structure domains:
Actin-related protein 2/3 complex subunit 1 Chains: C, H
Molecule details ›
Chains: C, H
Length: 377 amino acids
Theoretical weight: 41.64 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: P78774 (Residues: 1-377; Coverage: 100%)
Gene names: SPBC14C8.06, arc1, sop2
Sequence domains: WD domain, G-beta repeat
Structure domains: YVTN repeat-like/Quinoprotein amine dehydrogenase
Actin-related protein 2/3 complex subunit 2 Chains: D, I
Molecule details ›
Chains: D, I
Length: 317 amino acids
Theoretical weight: 37.03 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: O14241 (Residues: 1-317; Coverage: 100%)
Gene names: SPAC6F6.10c, arc2, arc34
Sequence domains: Arp2/3 complex, 34 kD subunit p34-Arc
Structure domains: Yope Regulator; Chain: A,
Actin-related protein 2/3 complex subunit 3 Chains: E, J
Molecule details ›
Chains: E, J
Length: 174 amino acids
Theoretical weight: 19.87 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: Q9Y7J4 (Residues: 1-174; Coverage: 100%)
Gene names: SPBC1778.08c, arc21, arc3
Sequence domains: ARP2/3 complex ARPC3 (21 kDa) subunit
Structure domains: Arc Repressor Mutant, subunit A
Actin-related protein 2/3 complex subunit 4 Chains: F, K
Molecule details ›
Chains: F, K
Length: 168 amino acids
Theoretical weight: 19.64 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: Q92352 (Residues: 1-168; Coverage: 100%)
Gene names: SPAC6G9.07c, arc4, arp20
Sequence domains: ARP2/3 complex 20 kDa subunit (ARPC4)
Structure domains: Yope Regulator; Chain: A,
Actin-related protein 2/3 complex subunit 5 Chains: G, L
Molecule details ›
Chains: G, L
Length: 152 amino acids
Theoretical weight: 16.92 KDa
Source organism: Schizosaccharomyces pombe
UniProt:
  • Canonical: Q10316 (Residues: 1-152; Coverage: 100%)
Gene names: SPAC17G8.04c, arc16, arc5
Sequence domains: ARP2/3 complex 16 kDa subunit (p16-Arc)
Structure domains: Actin-related protein 2/3 complex subunit 5

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P4222
Unit cell:
a: 218.967Å b: 218.967Å c: 315.049Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.325 0.324 0.344