3dsc

X-ray diffraction
2.7Å resolution

Crystal structure of P. furiosus Mre11 DNA synaptic complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA double-strand break repair protein Mre11 Chain: A
Molecule details ›
Chain: A
Length: 349 amino acids
Theoretical weight: 40.92 KDa
Source organism: Pyrococcus furiosus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U1N9 (Residues: 1-343; Coverage: 81%)
Gene names: PF1166, mre11
Sequence domains: Calcineurin-like phosphoesterase
Structure domains:
DNA (5'-D(P*DCP*DAP*DCP*DAP*DAP*DGP*DCP*DTP*DTP*DTP*DTP*DGP*DCP*DTP*DTP*DGP*DTP*DGP*DAP*DC)-3') Chain: B
Molecule details ›
Chain: B
Length: 20 nucleotides
Theoretical weight: 6.1 KDa
Source organism: Pyrococcus furiosus
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: C222
Unit cell:
a: 98.477Å b: 106.071Å c: 76.667Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.228 0.278
Expression systems:
  • Escherichia coli
  • Not provided