PDBe 3dib

X-ray diffraction
1.4Å resolution

Crystal structure of bovine pancreatic ribonuclease A variant (I106A)

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 124 amino acids
Theoretical weight: 13.67 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61823 (Residues: 27-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS FR590
Spacegroup: P3221
Unit cell:
a: 64.331Å b: 64.331Å c: 63.922Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.185 0.185 0.218
Expression system: Escherichia coli BL21(DE3)