PDBe 3deg

Electron Microscopy
10.9Å resolution

Complex of elongating Escherichia coli 70S ribosome and EF4(LepA)-GMPPNP

Released:
Source organism: Escherichia coli K-12
Related structures: EMD-1524

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 11-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
8 distinct RNA molecules
Macromolecules (11 distinct):
Elongation factor 4 Chain: C
Molecule details ›
Chain: C
Length: 545 amino acids
Theoretical weight: 60.48 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P60785 (Residues: 1-545; Coverage: 91%)
Gene names: JW2553, b2569, lepA
Sequence domains:
30S ribosomal protein S12 Chain: D
Molecule details ›
Chain: D
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
50S ribosomal protein L11 Chain: H
Molecule details ›
Chain: H
Length: 141 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli K-12
UniProt:
  • Canonical: P0A7J7 (Residues: 2-142; Coverage: 99%)
Gene names: JW3946, b3983, relC, rplK
Sequence domains:
P-tRNA Chain: B
Molecule details ›
Chain: B
Length: 77 nucleotides
Theoretical weight: 24.82 KDa
A/L-tRNA Chain: A
Molecule details ›
Chain: A
Length: 76 nucleotides
Theoretical weight: 24.89 KDa
30S RNA helix 8 Chain: E
Molecule details ›
Chain: E
Length: 16 nucleotides
Theoretical weight: 5.16 KDa
30S RNA helix 14 Chain: F
Molecule details ›
Chain: F
Length: 12 nucleotides
Theoretical weight: 3.83 KDa
50S RNA helix 71 Chain: J
Molecule details ›
Chain: J
Length: 18 nucleotides
Theoretical weight: 5.77 KDa
50S RNA helix 92 Chain: K
Molecule details ›
Chain: K
Length: 15 nucleotides
Theoretical weight: 4.75 KDa
50S RNA helix 95 Chain: I
Molecule details ›
Chain: I
Length: 29 nucleotides
Theoretical weight: 9.39 KDa
50S RNA helix 42-44 Chain: G
Molecule details ›
Chain: G
Length: 70 nucleotides
Theoretical weight: 22.58 KDa

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
Resolution: 10.9Å
Relevant EMDB volumes: EMD-1524
Expression system: Escherichia coli