PDBe 3daw

X-ray diffraction
2.55Å resolution

Structure of the actin-depolymerizing factor homology domain in complex with actin

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chain: A
Molecule details ›
Chain: A
Length: 377 amino acids
Theoretical weight: 42.1 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 1-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Structure domains:
Twinfilin-1 Chain: B
Molecule details ›
Chain: B
Length: 164 amino acids
Theoretical weight: 19.35 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91YR1 (Residues: 167-322; Coverage: 45%)
Gene names: Ptk9, Twf1
Sequence domains: Cofilin/tropomyosin-type actin-binding protein
Structure domains: Severin

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 52.78Å b: 72.99Å c: 168.88Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.212 0.208 0.279
Expression system: Escherichia coli