PDBe 3dap

X-ray diffraction
2.2Å resolution

C. GLUTAMICUM DAP DEHYDROGENASE IN COMPLEX WITH NADP+ AND THE INHIBITOR 5S-ISOXAZOLINE

Released:

Function and Biology Details

Reaction catalysed:
Meso-2,6-diaminoheptanedioate + H(2)O + NADP(+) = L-2-amino-6-oxoheptanedioate + NH(3) + NADPH
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Meso-diaminopimelate D-dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 320 amino acids
Theoretical weight: 35.24 KDa
Source organism: Corynebacterium glutamicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P04964 (Residues: 1-320; Coverage: 100%)
Gene names: Cgl2617, cg2900, ddh
Sequence domains: Diaminopimelic acid dehydrogenase C-terminal domain
Structure domains:

Ligands and Environments


Cofactor: Ligand NDP 2 x NDP
1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH2R
Spacegroup: P21
Unit cell:
a: 75.6Å b: 65.6Å c: 84.5Å
α: 90° β: 106.6° γ: 90°
R-values:
R R work R free
0.175 0.175 0.233
Expression system: Escherichia coli BL21(DE3)