3d4u

X-ray diffraction
1.7Å resolution

Bovine thrombin-activatable fibrinolysis inhibitor (TAFIa) in complex with tick-derived carboxypeptidase inhibitor.

Released:

Function and Biology Details

Reaction catalysed:
Release of C-terminal Arg and Lys from a polypeptide.
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Carboxypeptidase B2 Chain: A
Molecule details ›
Chain: A
Length: 309 amino acids
Theoretical weight: 36.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: Q2KIG3 (Residues: 115-423; Coverage: 77%)
Gene name: CPB2
Sequence domains: Zinc carboxypeptidase
Structure domains: Zn peptidases
Carboxypeptidase inhibitor Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 7.81 KDa
Source organism: Rhipicephalus bursa
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5EPH2 (Residues: 23-96; Coverage: 99%)
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P3221
Unit cell:
a: 84.2Å b: 84.2Å c: 127.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.156 0.156 0.171
Expression system: Escherichia coli