3d44

X-ray diffraction
1.9Å resolution

Crystal structure of HePTP in complex with a dually phosphorylated Erk2 peptide mimetic

Released:
Source organism: Homo sapiens

Function and Biology Details

Reactions catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 7 Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 35.2 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P35236 (Residues: 65-360; Coverage: 82%)
Gene name: PTPN7
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Mitogen-activated protein kinase 1 Chain: B
Molecule details ›
Chain: B
Length: 8 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P28482 (Residues: 184-191; Coverage: 2%)
Gene names: ERK2, MAPK1, PRKM1, PRKM2

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: C2
Unit cell:
a: 119.072Å b: 38.88Å c: 83.698Å
α: 90° β: 124.89° γ: 90°
R-values:
R R work R free
0.174 0.164 0.204
Expression systems:
  • Escherichia coli
  • Not provided