3cvw

X-ray diffraction
3.2Å resolution

Drosophila melanogaster (6-4) photolyase H365N mutant bound to ds DNA with a T-T (6-4) photolesion and cofactor F0

Released:
Source organism: Drosophila melanogaster
Entry authors: Maul MJ, Barends TRM, Glas AF, Cryle MJ, Schlichting I, Carell T

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Photolyase/cryptochrome alpha/beta domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 543 amino acids
Theoretical weight: 62.89 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8SXK5 (Residues: 1-520; Coverage: 96%)
Gene names: CG2488, phr6-4
Sequence domains:
Structure domains:
DNA (5'-D(*DAP*DCP*DAP*DGP*DCP*DGP*DGP*(64T)P*(5PY)P*DGP*DCP*DAP*DGP*DGP*DT)-3') Chain: C
Molecule details ›
Chain: C
Length: 15 nucleotides
Theoretical weight: 4.65 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided
DNA (5'-D(*DTP*DAP*DCP*DCP*DTP*DGP*DCP*DAP*DAP*DCP*DCP*DGP*DCP*DTP*DG)-3') Chain: D
Molecule details ›
Chain: D
Length: 15 nucleotides
Theoretical weight: 4.53 KDa
Source organism: Drosophila melanogaster
Expression system: Not provided

Ligands and Environments

2 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 86.47Å b: 89.51Å c: 89.34Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.275 0.272 0.329
Expression systems:
  • Escherichia coli
  • Not provided