› 3cs8

X-ray diffraction
2.3Å resolution

Structural and Biochemical Basis for the Binding Selectivity of PPARg to PGC-1a

Released:
DOI: 10.2210/pdb3cs8/pdb
PDB 3cs8 coloured by chain and viewed from the front.
PDB 3cs8 coloured by chain and viewed from the side.
PDB 3cs8 coloured by chain and viewed from the top.
Source organism: Homo sapiens
Primary publication:
Structural and biochemical basis for the binding selectivity of peroxisome proliferator-activated receptor gamma to PGC-1alpha.
Li Y, Kovach A, Suino-Powell K, Martynowski D, Xu HE
J. Biol. Chem. 283 19132-9 (2008)
PMID: 18469005

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Peroxisome proliferator-activated receptor gamma Chain: A
Molecule details ›
Chain: A
Length: 275 amino acids
Theoretical weight: 31.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P37231 (Residues: 234-504; Coverage: 54%)
Gene names: NR1C3, PPARG
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha Chain: B
Molecule details ›
Chain: B
Length: 12 amino acids
Theoretical weight: 1.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UBK2 (Residues: 141-152; Coverage: 2%)
Gene names: LEM6, PGC1, PGC1A, PPARGC1, PPARGC1A

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand BRL 1 x BRL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 32-ID
Spacegroup: C2
Unit cell:
a: 95.111Å b: 53.887Å c: 64.889Å
α: 90° β: 104.83° γ: 90°
R-values:
R R work R free
0.227 0.224 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Mode of action framework analysis for receptor-mediated toxicity: The peroxisome proliferator-activated receptor alpha (PPARĪ±) as a case study.
Corton et al. (2014)
1 more

9 mentions without citation

Predicting new indications for approved drugs using a proteochemometric method.
Dakshanamurthy et al. (2012)
8 more