3co6

X-ray diffraction
2.1Å resolution

Crystal Structure of FoxO1 DBD Bound to DBE1 DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Forkhead box protein O1 Chain: C
Molecule details ›
Chain: C
Length: 100 amino acids
Theoretical weight: 11.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12778 (Residues: 151-249; Coverage: 15%)
Gene names: FKHR, FOXO1, FOXO1A
Sequence domains: Forkhead domain
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA (5'-D(*DTP*DGP*DGP*DTP*DTP*DTP*DGP*DTP*DTP*DTP*DAP*DCP*DCP*DTP*DTP*DG)-3') Chain: A
Molecule details ›
Chain: A
Length: 16 nucleotides
Theoretical weight: 4.9 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*DCP*DAP*DAP*DGP*DGP*DTP*DAP*DAP*DAP*DCP*DAP*DAP*DAP*DCP*DCP*DA)-3') Chain: B
Molecule details ›
Chain: B
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X6A
Spacegroup: I222
Unit cell:
a: 65.518Å b: 76.44Å c: 102.143Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.211 0.256
Expression systems:
  • Escherichia coli
  • Not provided