3clz

X-ray diffraction
2.2Å resolution

The set and ring associated (SRA) domain of UHRF1 bound to methylated DNA

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
E3 ubiquitin-protein ligase UHRF1 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 212 amino acids
Theoretical weight: 23.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q96T88 (Residues: 413-617; Coverage: 26%)
Gene names: ICBP90, NP95, RNF106, UHRF1
Sequence domains: SAD/SRA domain
Structure domains: SRA-YDG
5'-D(*DGP*DGP*DGP*DCP*DCP*(5CM)P*DGP*DCP*DAP*DGP*DGP*DG)-3' Chains: E, G, I, K
Molecule details ›
Chains: E, G, I, K
Length: 12 nucleotides
Theoretical weight: 3.74 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(*DCP*DCP*DCP*DTP*DGP*DCP*DGP*DGP*DGP*DCP*DCP*DC)-3' Chains: F, H, J, L
Molecule details ›
Chains: F, H, J, L
Length: 12 nucleotides
Theoretical weight: 3.6 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P21
Unit cell:
a: 76.198Å b: 111.359Å c: 97.403Å
α: 90° β: 90.53° γ: 90°
R-values:
R R work R free
0.191 0.189 0.231
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided