3cii

X-ray diffraction
4.41Å resolution

Structure of NKG2A/CD94 bound to HLA-E

Released:
Source organism: Homo sapiens
Primary publication:
Structural basis for NKG2A/CD94 recognition of HLA-E.
Proc. Natl. Acad. Sci. U.S.A. 105 6696-701 (2008)
PMID: 18448674

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Soluble HLA class I histocompatibility antigen, alpha chain E Chains: A, D
Molecule details ›
Chains: A, D
Length: 273 amino acids
Theoretical weight: 31.64 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P13747 (Residues: 23-295; Coverage: 81%)
Gene names: HLA-6.2, HLA-E, HLAE
Sequence domains: Immunoglobulin C1-set domain
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Soluble HLA class I histocompatibility antigen, alpha chain G Chains: C, F
Molecule details ›
Chains: C, F
Length: 9 amino acids
Theoretical weight: 1.05 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P17693 (Residues: 3-11; Coverage: 0%)
Gene names: HLA-6.0, HLA-G, HLAG
Natural killer cells antigen CD94 Chains: G, I
Molecule details ›
Chains: G, I
Length: 121 amino acids
Theoretical weight: 14.21 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13241 (Residues: 59-179; Coverage: 68%)
Gene names: CD94, KLRD1
Sequence domains: Lectin C-type domain
NKG2-A/NKG2-B type II integral membrane protein Chains: H, J
Molecule details ›
Chains: H, J
Length: 120 amino acids
Theoretical weight: 13.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P26715 (Residues: 113-232; Coverage: 52%)
Gene names: KLRC1, NKG2A
Sequence domains: Lectin C-type domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: I4132
Unit cell:
a: 345.983Å b: 345.983Å c: 345.983Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.321 0.321 0.355
Expression systems:
  • Escherichia coli
  • Not provided