PDBe 3cbm

X-ray diffraction
1.69Å resolution

SET7/9-ER-AdoMet complex

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase SETD7 Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.56 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WTS6 (Residues: 111-366; Coverage: 70%)
Gene names: KIAA1717, KMT7, SET7, SET9, SETD7
Sequence domains: SET domain
Structure domains:
Estrogen receptor Chain: B
Molecule details ›
Chain: B
Length: 10 amino acids
Theoretical weight: 1.16 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-BM
Spacegroup: P21212
Unit cell:
a: 102.338Å b: 38.902Å c: 66.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.196 0.229
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided