› 3c98

X-ray diffraction
2.6Å resolution

Revised structure of the munc18a-syntaxin1 complex

Released:
DOI: 10.2210/pdb3c98/pdb
PDB 3c98 coloured by chain and viewed from the front.
PDB 3c98 coloured by chain and viewed from the side.
PDB 3c98 coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Munc18a controls SNARE assembly through its interaction with the syntaxin N-peptide.
Burkhardt P, Hattendorf DA, Weis WI, Fasshauer D
EMBO J 27 923-33 (2008)
PMID: 18337752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Syntaxin-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 606 amino acids
Theoretical weight: 69.07 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61765 (Residues: 1-594; Coverage: 100%)
Gene names: Stxbp1, Unc18a
Sequence domains: Sec1 family
Structure domains:
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 279 amino acids
Theoretical weight: 32.45 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 1-267; Coverage: 93%)
Gene names: Sap, Stx1a
Sequence domains:
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P4212
Unit cell:
a: 158.07Å b: 158.07Å c: 81.45Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.203 0.263
Expression system: Escherichia coli

Quick links

Citations

26 review citations

Synaptic vesicle fusion.
Rizo et al. (2008)
25 more

61 mentions without citation

At the junction of SNARE and SM protein function.
Carr et al. (2010)
60 more