PDBe 3c1g

X-ray diffraction
2.3Å resolution

Substrate binding, deprotonation and selectivity at the periplasmic entrance of the E. coli ammonia channel AmtB

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ammonia channel Chain: A
Molecule details ›
Chain: A
Length: 424 amino acids
Theoretical weight: 44.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P69681 (Residues: 23-428; Coverage: 100%)
Gene names: JW0441, amtB, b0451, ybaG
Sequence domains: Ammonium Transporter Family
Structure domains: Ammonium transporter AmtB like domains

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P63
Unit cell:
a: 109.329Å b: 109.329Å c: 84.821Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.185 0.242
Expression system: Escherichia coli