3bwu

X-ray diffraction
1.76Å resolution

Crystal structure of the ternary complex of FimD (N-Terminal Domain, FimDN) with FimC and the N-terminally truncated pilus subunit FimF (FimFt)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Chaperone protein FimC Chain: C
Molecule details ›
Chain: C
Length: 205 amino acids
Theoretical weight: 22.75 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31697 (Residues: 37-241; Coverage: 100%)
Gene names: JW4279, b4316, fimC
Sequence domains:
Structure domains: Immunoglobulins
Outer membrane usher protein FimD Chain: D
Molecule details ›
Chain: D
Length: 125 amino acids
Theoretical weight: 13.66 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30130 (Residues: 46-170; Coverage: 15%)
Gene names: JW5780, b4317, fimD
Structure domains: Ubiquitin-like (UB roll)
Protein FimF Chain: F
Molecule details ›
Chain: F
Length: 142 amino acids
Theoretical weight: 14.84 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P08189 (Residues: 35-176; Coverage: 91%)
Gene names: JW4281, b4318, fimF
Structure domains: Fimbrial-type adhesion domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P3121
Unit cell:
a: 126.948Å b: 126.948Å c: 68.755Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.175 0.212
Expression system: Escherichia coli