› 3bt2

X-ray diffraction
2.5Å resolution

Structure of urokinase receptor, urokinase and vitronectin complex

Released:
DOI: 10.2210/pdb3bt2/pdb
PDB entry 3bt2 coloured by chain, front view.
PDB entry 3bt2 coloured by chain, side view.
PDB entry 3bt2 coloured by chain, top view.
Source organisms:
Primary publication:
Crystal structures of two human vitronectin, urokinase and urokinase receptor complexes.
Huai Q, Zhou A, Lin L, Mazar AP, Parry GC, Callahan J, Shaw DE, Furie B, Furie BC, Huang M
Nat Struct Mol Biol 15 422-3 (2008)
PMID: 18376415

Function and Biology Details

Reaction catalysed: 
Specific cleavage of Arg-|-Val bond in plasminogen to form plasmin.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero tetramer
monomeric
Assembly name:
PDBe Complex ID:
PDB-CPX-233879 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (6 distinct):
Urokinase-type plasminogen activator long chain A Chain: A
Molecule details ›
Chain: A
Length: 135 amino acids
Theoretical weight: 15.36 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00749 (Residues: 21-153; Coverage: 32%)
Gene name: PLAU
Sequence domains: Kringle domain
Structure domains:
Somatomedin-B Chain: B
Molecule details ›
Chain: B
Length: 40 amino acids
Theoretical weight: 4.57 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P04004 (Residues: 21-60; Coverage: 9%)
Gene name: VTN
Sequence domains: Somatomedin B domain
anti-uPAR antibody, light chain Chain: L
Molecule details ›
Chain: L
Length: 212 amino acids
Theoretical weight: 23.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
anti-uPAR antibody, heavy chain Chain: H
Molecule details ›
Chain: H
Length: 214 amino acids
Theoretical weight: 23.04 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
Structure domains: Immunoglobulins
Urokinase plasminogen activator surface receptor Chain: U
Molecule details ›
Chain: U
Length: 283 amino acids
Theoretical weight: 31.6 KDa
Source organism: Homo sapiens
Expression system: Drosophila melanogaster
UniProt:
  • Canonical: Q03405 (Residues: 23-303; Coverage: 90%)
Gene names: MO3, PLAUR, UPAR
Sequence domains: u-PAR/Ly-6 domain
Structure domains: CD59

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C, NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 52.096Å b: 87.205Å c: 124.274Å
α: 90° β: 94.31° γ: 90°
R-values:
R R work R free
0.23 0.229 0.272
Expression systems:
  • Escherichia coli BL21
  • Escherichia coli
  • Drosophila melanogaster

Quick links

Citations

15 review citations

New insights into the molecular mechanisms of the fibrinolytic system.
Rijken et al. (2009)
14 more

12 mentions without citation

uPAR: An Essential Factor for Tumor Development.
Lv et al. (2021)
11 more