X-ray diffraction
2.2Å resolution

NEMO/IKKb association domain structure


Function and Biology Details

Reactions catalysed:
ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Inhibitor of nuclear factor kappa-B kinase subunit beta Chains: A, C
Molecule details ›
Chains: A, C
Length: 48 amino acids
Theoretical weight: 5.44 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: O14920 (Residues: 701-745; Coverage: 6%)
Gene names: IKBKB, IKKB
Sequence domains: I-kappa-kinase-beta NEMO binding domain
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
NF-kappa-B essential modulator Chains: B, D
Molecule details ›
Chains: B, D
Length: 70 amino acids
Theoretical weight: 8.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q9Y6K9 (Residues: 44-111; Coverage: 16%)
Gene names: FIP3, IKBKG, NEMO
Sequence domains: NF-kappa-B essential modulator NEMO
Structure domains: L1 transposable element, trimerization domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 44.82Å b: 50.67Å c: 102.79Å
α: 90° β: 90° γ: 90°
R R work R free
0.212 0.208 0.294
Expression systems:
  • Not provided
  • Escherichia coli BL21(DE3)