X-ray diffraction
2.25Å resolution

NEMO/IKK association domain structure


Function and Biology Details

Reactions catalysed:
ATP + [I-kappa-B protein] = ADP + [I-kappa-B phosphoprotein]
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
hetero tetramer
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Inhibitor of nuclear factor kappa-B kinase subunit alpha; Inhibitor of nuclear factor kappa-B kinase subunit beta Chains: A, C
Molecule details ›
Chains: A, C
Length: 47 amino acids
Theoretical weight: 5.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: O15111 (Residues: 732-745; Coverage: 2%)
  • Canonical: O14920 (Residues: 701-730; Coverage: 4%)
Gene names: CHUK, IKBKB, IKKA, IKKB, TCF16
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
NF-kappa-B essential modulator Chains: B, D
Molecule details ›
Chains: B, D
Length: 68 amino acids
Theoretical weight: 8.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: Q9Y6K9 (Residues: 44-111; Coverage: 16%)
Gene names: FIP3, IKBKG, NEMO
Sequence domains: NF-kappa-B essential modulator NEMO
Structure domains: L1 transposable element, trimerization domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 41.869Å b: 105.556Å c: 56.707Å
α: 90° β: 90° γ: 90°
R R work R free
0.24 0.238 0.282
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided