X-ray diffraction
2.2Å resolution

Protein Tyrosine Phosphatase PTPN-22 (Lyp) bound to the mono-Phosphorylated Lck active site peptide

Source organism: Homo sapiens
Entry authors: Seidel RD, Love J, Piserchio A, Cowburn D

Function and Biology Details

Reactions catalysed:
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Tyrosine-protein phosphatase non-receptor type 22 Chains: A, B
Molecule details ›
Chains: A, B
Length: 310 amino acids
Theoretical weight: 36.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: Q9Y2R2 (Residues: 1-310; Coverage: 38%)
Gene names: PTPN22, PTPN8
Sequence domains: Protein-tyrosine phosphatase
Structure domains: Protein tyrosine phosphatase superfamily
Tyrosine-protein kinase Lck Chains: C, D
Molecule details ›
Chains: C, D
Length: 7 amino acids
Theoretical weight: 869 Da
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P06239 (Residues: 391-397; Coverage: 1%)
Gene name: LCK

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P21
Unit cell:
a: 60.85Å b: 48.51Å c: 119.97Å
α: 90° β: 103.49° γ: 90°
R R work R free
0.191 0.188 0.264
Expression systems:
  • Escherichia coli
  • Not provided