PDBe 3brg

X-ray diffraction
2.2Å resolution

CSL (RBP-Jk) bound to DNA

Released:
Source organism: Mus musculus

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Recombining binding protein suppressor of hairless Chain: C
Molecule details ›
Chain: C
Length: 427 amino acids
Theoretical weight: 48.28 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P31266 (Residues: 53-474; Coverage: 80%)
Gene names: Igkjrb1, Igkrsbp, Rbpj, Rbpsuh
Sequence domains:
Structure domains:
DNA (5'-D(*DAP*DAP*DTP*DCP*DTP*DTP*DTP*DCP*DCP*DCP*DAP*DCP*DAP*DGP*DT)-3') Chain: A
Molecule details ›
Chain: A
Length: 15 nucleotides
Theoretical weight: 4.5 KDa
Source organism: Mus musculus
Expression system: Not provided
DNA (5'-D(*DTP*DTP*DAP*DCP*DTP*DGP*DTP*DGP*DGP*DGP*DAP*DAP*DAP*DGP*DA)-3') Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.67 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P21212
Unit cell:
a: 66.769Å b: 95.388Å c: 113.695Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.255
Expression systems:
  • Escherichia coli
  • Not provided