› 3bpn

X-ray diffraction
3.02Å resolution

Crystal structure of the IL4-IL4R-IL13Ra ternary complex

Released:
DOI: 10.2210/pdb3bpn/pdb
PDB entry 3bpn coloured by chain, front view.
PDB entry 3bpn coloured by chain, side view.
PDB entry 3bpn coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Molecular and structural basis of cytokine receptor pleiotropy in the interleukin-4/13 system.
LaPorte SL, Juo ZS, Vaclavikova J, Colf LA, Qi X, Heller NM, Keegan AD, Garcia KC
Cell 132 259-72 (2008)
PMID: 18243101

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-138512 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-4 Chain: A
Molecule details ›
Chain: A
Length: 129 amino acids
Theoretical weight: 14.99 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P05112 (Residues: 25-153; Coverage: 100%)
Gene name: IL4
Sequence domains: Interleukin 4
Structure domains: Growth Hormone; Chain: A;
Soluble interleukin-4 receptor subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 205 amino acids
Theoretical weight: 23.47 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P24394 (Residues: 27-227; Coverage: 25%)
Gene names: 582J2.1, IL4R, IL4RA
Sequence domains: Interleukin-4 receptor alpha chain, N-terminal
Structure domains: Immunoglobulins
Interleukin-13 receptor subunit alpha-1 Chain: C
Molecule details ›
Chain: C
Length: 314 amino acids
Theoretical weight: 36.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P78552 (Residues: 29-342; Coverage: 77%)
Gene names: IL13R, IL13RA, IL13RA1
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 61.64Å b: 62.841Å c: 115.125Å
α: 90° β: 96.31° γ: 90°
R-values:
R R work R free
0.238 0.235 0.304
Expression system: Spodoptera frugiperda

Quick links

Citations

105 review citations

Alternative activation of macrophages: mechanism and functions.
Gordon et al. (2010)
104 more

11 mentions without citation

Molecular basis for shared cytokine recognition revealed in the structure of an unusually high affinity complex between IL-13 and IL-13Ralpha2.
Lupardus et al. (2010)
10 more