PDBe 3blw

X-ray diffraction
4.3Å resolution

Yeast Isocitrate Dehydrogenase with Citrate and AMP Bound in the Regulatory Subunits

Released:
Source organism: Saccharomyces cerevisiae

Function and Biology Details

Reaction catalysed:
Isocitrate + NAD(+) = 2-oxoglutarate + CO(2) + NADH
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero octamer
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 349 amino acids
Theoretical weight: 38.08 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28834 (Residues: 12-360; Coverage: 97%)
Gene names: IDH1, N2690, YNL037C
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Isocitrate dehydrogenase [NAD] subunit 2, mitochondrial Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 354 amino acids
Theoretical weight: 37.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P28241 (Residues: 16-369; Coverage: 96%)
Gene names: IDH2, O3326, YOR136W, YOR3326W
Sequence domains: Isocitrate/isopropylmalate dehydrogenase

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P1
Unit cell:
a: 113.157Å b: 116.346Å c: 163.62Å
α: 98.96° β: 110.23° γ: 106.63°
R-values:
R R work R free
0.276 0.274 0.311
Expression system: Escherichia coli BL21(DE3)