PDBe 3bl6

X-ray diffraction
1.7Å resolution

Crystal structure of Staphylococcus aureus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase in complex with formycin A

Released:
Source organism: Staphylococcus aureus
Primary publication:
Structure of Staphylococcus aureus 5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 64 343-50 (2008)
PMID: 18453700

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-homocysteine + H(2)O = S-(5-deoxy-D-ribos-5-yl)-L-homocysteine + adenine
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
5'-methylthioadenosine/S-adenosylhomocysteine nucleosidase Chain: A
Molecule details ›
Chain: A
Length: 230 amino acids
Theoretical weight: 24.76 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q99TQ0 (Residues: 1-228; Coverage: 100%)
Gene names: SAV1599, mtnN
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 58.25Å b: 81.67Å c: 45.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.187
Expression system: Escherichia coli BL21(DE3)